泵用异步电动机直接转矩控制系统的设计

直接转矩控制（DTC）是一种高性能的控制方法，提出了一种基于TMS320C32的直接转矩控制系统实现方案。通过硬和软件设计，实现对异步电动机的直接转矩控制，实验结果表明了该方案的可行性，将其应用于水泵的调速系统中，能进一步提高水泵的运行效率。     

QZS45型汽车驱动桥总成在线检测系统的研制

针对汽车驱动桥总成生产中在线检测手段落后的现状,研制了QZS45型汽车驱动桥总成在线测试系统。该系统选用了隔离的静音检测室,采用计算机控制、变频调速电动机驱动;系统中的转矩转速、温度、噪声等测量信号通过板卡与计算机连接,通过编制控制软件,保证了在线检测工作的顺利进行,并实现了自动采样、自动判断、自动汇总的功能,满足了汽车驱动桥总成在线检测的各项要求。     

辽宁省遭遇农产品技术贸易壁垒的特点及对策

为提高辽宁省农产品在国际市场上的竞争力,简述美国、日本、欧盟等发达国家实施壁垒的情况和WTO的相关规定,根据辽宁省农产品出口遭遇的技术贸易壁垒在检测指标、涉及范围、连锁反应、控制点4方面的变化趋势,提出切实可行的应对措施。     

小波分析在拖拉机齿轮箱故障诊断中的应用

在分析了采用傅立叶变换对拖拉机齿轮箱故障诊断进行信号分析技术所存在的不足的基础上，提出了采用小波变换对该信号进行分析处理时，基小波选取的方法。同时在对拖拉机齿轮箱故障诊断的实际应用中，用齿轮箱振动的加速度信号对傅立叶变换和小波变换的结果进行了比较，证明了应用小波变换能准确反映齿轮箱故障的结果，并提出用小波变换可以建立故障自动诊断系统。     

数字化电机调速系统中神经元控制器的应用

神经网络具有自学习、自适应能力，用于控制时可不依赖控制对象的数学模型。为此，基于单神经元设计出用于交流电机矢量控制的自适应磁链和转速控制器，并应用于由数字信号处理器(DSP)实现的交流电机矢量控制系统中，实验表明：此方法设计的控制器结构简单，易于数字化实现，控制系统动态性能良好。     

STATIC AND DYNAMIC 18FDG‐PET IN NORMAL HISPANIOLAN AMAZON PARROTS (AMAZONA VENTRALIS)

Positron emission tomography (PET) is often used to stage and monitor human cancer and has recently been used in a similar fashion in veterinary medicine. The most commonly used radiopharmaceutical is 2‐Deoxy‐2‐[¹⁸F]‐Fluoro‐d ‐glucose (¹⁸F‐FDG), which is concentrated and trapped within cells that use glucose as their energy substrate. We characterized the normal distribution of ¹⁸F‐FDG in 10 healthy Hispaniolan Amazon parrots (Amazona ventralis) by performing whole body PET scans at steady state, 60 min after injection. Significant variability was found in the intestinal activity. Avian species are known to reflux fluid and electrolytes from their cloaca into their colon. To evaluate reflux as the cause of variability in intestinal distribution of ¹⁸F‐FDG, dynamic PET scans were performed on the coelomic cavity of six Hispaniolan Amazon parrots from time 0 to 60 min postinjection of radiotracer. Reflux of radioactive material from the cloaca into the colon occurred in all birds to varying degrees and occurred before 60 min. To evaluate the intestinal tract of clinical avian patients, dynamic scans must be performed starting immediately after injection so that increased radioactivity due to metabolism or hypermetabolic lesions such as cancer can be differentiated from increased radioactivity due to reflux of fluid from the cloaca.     

Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid

Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the blastocysts were transferred into the uterine horn of estrus-synchronized recipients. SCNT embryos that were cultured in mSOF or CDM developed to the blastocysts stages at similar rates (26.6% vs. 22.5%, respectively). A total of 67 preimplantational stage embryos were transferred into 34 recipients and six cloned calves were born by caesarean section, or assisted or natural delivery. Survival of transferred blastocysts to live cloned calves in the mSOF and the CDM was 18.5% (to recipients), 9.6% (to blastocysts) and 42.9% (to recipients), 20.0% (to blastocysts), respectively. DNA analysis showed that all cloned calves were genetically identical to the donor cells. These results demonstrate that SCNT embryos cultured in CDM showed higher viability as judged by survival of the calves that came to term compared to blastocysts derived from mSOF cultures.     

In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle''s salt with L-glutamine and sodium bicarbonate) for 27 h at 39℃ under 5% CO₂ in humidified air. The oocytes retrieval and efficiency (mean ± SD) per prepubertal and pubertal goats were 5.2 ± 0.6 and 6.8 ± 0.6, and 77.3 ± 0.1% and 80.5 ± 0.6%, respectively. Anaphase I - telophase I stages differed significantly (7.3 ± 0.8 vs. 2.6 ± 0.2, p < 0.05) between the two groups of goats. After IVM, the percentages of metaphase II were significantly higher (66.3 vs. 60.3, p < 0.05) in pubertal goats than in their prepubertal counterparts. The percentages of normal in vitro fertilization (IVF) in Fert-Tyrode''s albumin lactate pyruvate of pubertal goat oocytes did not differ between Percoll and swim-up sperm separation methods (36.7 ± 0.9% vs. 32.7 ± 1.3%, p > 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production.     

DNA methylation analysis on satellite I region in blastocysts obtained from somatic cell cloned cattle

Many observations have been made on cloned embryos and on adult clones by somatic cell nuclear transfer (SCNT), but it is still unclear whether the progeny of cloned animals is presenting normal epigenetic status. Here, in order to accumulate the information for evaluating the normality of cloned cattle, we analyzed the DNA methylation status on satellite I region in blastocysts obtained from cloned cattle. Embryos were produced by artificial insemination (AI) to non‐cloned or cloned dams using semen from non‐cloned or cloned sires. After 7 days of AI, embryos at blastocyst stage were collected by uterine flushing. The DNA methylation levels in embryos obtained by using semen and/or oocytes from cloned cattle were similar to those in in vivo embryos from non‐cloned cattle. In contrast, the DNA methylation levels in SCNT embryos were significantly higher (P < 0.01) than those in in vivo embryos from non‐cloned and cloned cattle, approximately similar to those in somatic cells used as donor cells. Thus, this study provides useful information that epigenetic status may be normal in the progeny of cloned cattle, suggesting the normality of germline cells in cloned cattle.     

植物细胞H_2O_2的产生及其生理作用

过氧化氢（hydrogen peroxide,H2O2）是细胞有氧代谢的产物,激素等发育信号和胁迫刺激都可以诱导细胞内H2O2的产生和积累。H2O2在细胞内浓度的升高和降低以及变化模式可能介导了不同的信号转导途径,并调控相应生长发育、胁迫应答等生物学过程。